Erratum
In the original article by Lee, et al., entitled „Expressional and Functional Characterization of
Intracellular pH Regulators and Effetcs of Ethanol in Human Oral Epidermoid Carcinoma Cells“
[Cell Physiol Biochem 2018;47:2056-2068, DOI: 10.1159/000491473], there have been made
several mistakes in the manuscript and results as well as a mistake in Fig. 7A.
The corrected versions of the respective sentences are displayed below, together with their
respective page number. The corrected Fig. 7 is also displayed below.
The authors sincerely apologize for this mistake.
On page 2057: „Such low pHe inside solid tumors is mainly attributable to the pHi extruders,
such as Na+-H+ exchanger (NHE) and Na+-HCO3- cotransporter (NBC) [6, 7].“
On page 2059: „Fig. 1A showed the emission ratio observed on perfusion with five different pH
calibration solutions (5.5˜8.5) in OEC-M1 cells. The maximum and minimal emitted ratio (Rmax
and Rmin) of 530nm at 490nm and 440nm excitations were obtained from perfusion with pH 8.5
and 5.5 calibration solution, respectively.“
On page 2060, Figure Legend 1: „In situ calibration of BCECF fluorescent dye, resting pHi and
kinetic steady-state pHi in OEC-M1 cells. (A) The trace shows the BCECF fluorescence (530 nm
emission at 490 nm and 440 nm excitations) that calibrated by superfusing with 5 different pH
(5.5-8.5) of nigericin solutions in OEC-M1 cells. (B) The curve and equation show the relationship
of BCECF fluorescence ratio and pHi that averaged from 5 similar experiments as shown in A. [...]“
On pages 2060-2061: „Our present results showed that ethanol (10-1000 mM) showed a
oncentration-dependent inhibitory effect on resting pHi (-0.01˜-0.05 pHi unit) in HEPES-buffered
solution, while a concentration-dependent and biphasic effect on resting pHi (+0.01˜-0.04 pHi
unit) in CO2/HCO3--buffered condition, as shown in histograms of Fig. 4E.“
On page 2063, Figure Legend 7: „Effect of chronic treatment of ethanol (24 hr) on isoforms
of acid extruders in OEC-M1 cells. (A) The western blot results show the changes of isoforms
of NHE1, NBCe1, NBCn1, NDCBE and β-actin expression (marked at the most left) after 24 hr
treatment with ethanol (10 to 1000 mM; marked at the top) in OEC-M1 cells. [...]“
On page 2064: „The ethanol-induced changes on expression of acid extruders, i.e. NHE and NBC
isoforms (Fig. 7), might be the reason to account for the higher extend on pHi changes upon 24 h
ethanol treatment.“